tat Exon 1 Exhibits Functional Diversity during HIV-1 Subtype C Primary Infection

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tat Exon 1 exhibits functional diversity during HIV-1 subtype C primary infection.

Human immunodeficiency virus type 1 (HIV-1) Tat is a mediator of viral transcription and is involved in the control of virus replication. However, associations between HIV-1 Tat diversity and functional effects during primary HIV-1 infection are still unclear. We estimated selection pressures in tat exon 1 using the mixed-effects model of evolution with 672 viral sequences generated from 20 pat...

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Viral Diversity and Diversification of Major Non-Structural Genes vif, vpr, vpu, tat exon 1 and rev exon 1 during Primary HIV-1 Subtype C Infection

To assess the level of intra-patient diversity and evolution of HIV-1C non-structural genes in primary infection, viral quasispecies obtained by single genome amplification (SGA) at multiple sampling timepoints up to 500 days post-seroconversion (p/s) were analyzed. The mean intra-patient diversity was 0.11% (95% CI; 0.02 to 0.20) for vif, 0.23% (95% CI; 0.08 to 0.38) for vpr, 0.35% (95% CI; -0...

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Timing Constraints of In Vivo Gag Mutations during Primary HIV-1 Subtype C Infection

BACKGROUND Aiming to answer the broad question "When does mutation occur?" this study examined the time of appearance, dominance, and completeness of in vivo Gag mutations in primary HIV-1 subtype C infection. METHODS A primary HIV-1C infection cohort comprised of 8 acutely and 34 recently infected subjects were followed frequently up to 500 days post-seroconversion (p/s). Gag mutations were ...

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Immunodynamics of Th17 cells in HIV-1 subtype ‘C’ infection

Methods 31 HIV seropositive antiretroviral therapy naïve and 8 HIV uninfected healthy control subjects were recruited and characterized as being early, late or slow progressor. Peripheral blood mononuclear cells were isolated from each study subject and stimulated with HIV-1 subtype ‘C’ gag peptide pool and assessed for IL-17 cytokine producing CD4-T cells using intracellular cytokine staining....

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ژورنال

عنوان ژورنال: Journal of Virology

سال: 2013

ISSN: 0022-538X

DOI: 10.1128/jvi.03297-12